Analysis of avian influenza among occupational exposure crowd in Ningxia from 2011 to 2013

Objective To learn the H5N1 avian influenza virus antibody level in occupational exposure crowd and avian influenza virus distribution in environment in Ningxia,so as to provide scientific basis for prevention and control of avian influenza. Methods Hemagglutination inhibition test was used to detect the A( H5N1) hemagglutinin antibody in occupational exposure crowd,while real-time RT-PCR was employed to detect H5,H7,H9 avian influenza virus nucleic acid in environmental samples. From April 2013,H7N9 nucleic acid was detected in environmental samples. Results All the 812 serum H5N1 antibodies from occupational exposure population were negative,while the positive rate of avian influenza A virus in 760 environmental specimens was 5. 13%. Except Yuanzhou,avian influenza A virus nucleic acid was positive in all of the other monitoring regions. Monitoring results of workplace environmental samples showed that the highest positive rate of avian influenza virus nucleic acid was found in live poultry markets of urban and rural areas. Avian influenza virus nucleic acid test results of different environment samples showed that the highest positive rate was found on surface of chopping board for placing poultry meat. Conclusion Antibody of A( H5N1) avian influenza virus was not found in occupational exposure crowd in Ningxia. In environment samples,avian influenza virus H5 and H9 were positive,but H7N9 was not detected. Live poultry markets in urban and rural areas is an important distribution center for avian influenza virus.