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Antibacterial effect of three inorganic nano-antibacterial agents against Enterococcus faecalis

Guo-rong Ye

Abstract

AIM: To evaluate the antibacterial effect of nano-silver,Ag-embedded nano-TiO2 and nitrogen-doped nano-TiO2 against E.faecalis.METHODS: E.faecalis was inoculated in MBECTM PG Assay to construct an E.faecalis infection model.E.faecalis infection on MBECTM PG Assay was observed by scaning electron microscope.The minimum inhibitory concentration(MIC) and minimum biofilm eradication concentration(MBEC) were detected by serial dilution method using MBECTM PG Assay.RESULTS: E.faecalis biofilm formed on MBECTM PG Assay.MIC and MBEC of nano-silver against E.faecalis were 62.5 mg/L and 1,000 mg/L respectively.MIC of Ag-embedded nano-TiO2 against E.faecalis was 64 g/L.MBEC of Ag-embedded nano-TiO2,MIC and MBEC of nitrogen-doped nano-TiO2 could not be detected in this study.MIC and MBEC of sodium hypochlorite were 6.56 g/L and 13.13 g/L.CONCLUSION: Nano-silver has strong antibacterial effect on E.faecalis and can eradicate E.faecalis biofilm.E.faecalis shows resistance against Ag-embedded nano-TiO2 and nitrogen-doped nano-TiO2.

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Key concepts: Enterococcus faecalis, Minimum inhibitory concentration, Biofilm, Microbiology, Minimum bactericidal concentration, Chemistry, Antibacterial activity, Sodium hypochlorite