Determination of cyclic 3′,5′‐adenosine monophosphate in plant tissues by high performance liquid chromatography

Abstract A quantitative method has been developed for determining cyclic 3′,5′‐adenosine monophosphate (cAMP) in plant tissues based on the use of high performance liquid chromatography following partial purification of cyclonucleotides from plant extracts by different chromatographic procedures. The identical nature of a plant cAMP‐like substance and synthetic cAMP has been demonstrated. The lower limit of cAMP detection was 2.6 ng, the overall efficiency of determination was 34.0% and the standard deviation was ±5.2%. The concentration of cAMP in rye seedlings was determined to be 0.700±0.069 n mol per g fresh weight of tissue.