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Expression of Human Bone Morphogenetic Protein 7 in Chinese Hamster Ovary Cells

Yide Huang

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Abstract

Objective:Construction of the recombinant human bone morphogenetic protein 7(rhBMP7) expression vector and expression of rhBMP7 in Chinese Hamster Ovary(CHO) cells.Method:The expression vectors containing hBMP7 cDNA was transformed into CHO cells by electroporation and expression levels of rhBMP7 was analyzed by Dot blot and ELISA.Result:hBMP7 cDNA was integrated into genome of CHO cells and was transcribed in recombinant cells.Expression of rhBMP7 was con-firmed by Dot blot and ELISA.Conclusion:hBMP7 was successfully expressed in CHO expression system.

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What this paper is about

Objective:Construction of the recombinant human bone morphogenetic protein 7(rhBMP7) expression vector and expression of rhBMP7 in Chinese Hamster Ovary(CHO) cells.Method:The expression vectors containing hBMP7 cDNA was transformed into CHO cells by electroporation and expression levels of rhBMP7 was analyzed by Dot blot and ELISA.Result:hBMP7 cDNA was integrated into genome of CHO cells and was transcribed in recombinant cells.Expression of rhBMP7 was con-firmed by Dot blot and ELISA.Conclusion:hBMP7 was successfully expressed in CHO expression system.

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Available abstract

Objective:Construction of the recombinant human bone morphogenetic protein 7(rhBMP7) expression vector and expression of rhBMP7 in Chinese Hamster Ovary(CHO) cells.Method:The expression vectors containing hBMP7 cDNA was transformed into CHO cells by electroporation and expression levels of rhBMP7 was analyzed by Dot blot and ELISA.Result:hBMP7 cDNA was integrated into genome of CHO cells and was transcribed in recombinant cells.Expression of rhBMP7 was con-firmed by Dot blot and ELISA.Conclusion:hBMP7 was successfully expressed in CHO expression system.

Key concepts: Chinese hamster ovary cell, Electroporation, Recombinant DNA, Molecular biology, Western blot, Complementary DNA, Transfection, Expression vector

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