The Effect of Calcitonin Gene Related Peptide and Nerve Growth Factor on the Expression of c-Fos in Striate Cortex Vessels of Rats with Transient Cerebral Ischemia/Reperfusion

Objective To study the effect of calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of c-Fos protein in striate cortex vessels of rats with transient cerebral ischemia/reperfusion(I/R).Methods Immunohistochemistry and microimage analysis were employed.Results The expression of c-Fos proteins in vessels and paravessels of striate cortex was absent in sham group,I/R group,NGF group,CGRP group and in NGF+CGRP group with 3h and 1d survival after I/R,and still without c-Fos expression 3 days after I/R.However,when the animals survived 3 days after I/R,the expression of c-Fos was increased markedly in vessels and paravessels of striate cortex in NGF group and CGRP group compared with I/R group,and much higher in NGF+CGRP group than in NGF group and CGRP group respectively.Conclusion The increased expression of c-Fos by CGRP and NGF respectively and higher increased c-Fos expression by combination of NGF and CGRP indicate that CGRP and NGF have coordinate protective effects on the ischemia neurons in brain.