Study on the Favorable Conditions of Silkworm Chrysalis's Chitosan

Better preparation of the silkworm chitin chitosan deacetylation conditions were studied by orthogonal.The results revealed that the sodium hydroxide concentration is the most important factor on the degree of the silkworm chitin chitosan deacetylation,followed by the temperature of treatment,processing time and solid to liquid ratio has little effect.After optimizing,the treatment conditions are: NaOH's concentration is 50%,treatment temperature is 90℃,time is 10h,solid-liquid ratio is 1:15.After validation test,under these conditions,the pupa obtained degree of deacetylation of chitosan up to 72%.