Analysis of Genetic Polymorphisms for κ-CN Gene 5′ and 3′ Flanking Region in Bovine

The study aims to detect polymorphisms of partial 5′ and 3′ flanking region of κ-CN gene by PCR-SSCP,the results showed that 5′ and 3′ flanking region of κ-CN gene were polymorphic.The allele frequencies of A,B of 5′ flanking region(AA,BB,AB) and 3′ flanking region(GG,GT) of κ-CN gene were 0.5345,0.4655 and 0.8621,0.1379,respectively.The 5′ flanking region was significantly deviated from Hardy-Weinberg equilibrium(P0.01),3′ flanking region was in agreement with the Hardy-Weinberg equilibrium(P0.05).Polymorphism of 5′ flanking region showed moderate polymorphism with PIC ranged from 0.25 to 0.5 while 3′ flanking region locus showed low polymorphism with PIC less than 0.5.The heterozygosity and number of effective alleles were 0.5019,0.2399 and 1.9905,1.3120,respectively in 5′ and 3′ flanking region of κ-CN gene of Holstein cattle.The research suggested that 5′ flanking region of κ-CN gene is more suitable to analyse the relationship between polymorphism and production performance,and used in the study of the locus of quantitative genetic.