Study on Determination of Volatile Phenol in Fish by Interval Flow Injection Analyzer

[Objective]To explore methods of measuring volatile phenol in fish by interval flow injection analyzer and researches of pollution in various fish. [Methods]After soaking in sodium hydroxide solution overnight, the fish was distilled. The distillate were diluted after collecting and filtering, and then determined by interval flow injection analyzer. [Results]Mass concentration of volatile phenol kept linear relationship with the absorbance within 100 μg/L. Method detect limit was 1.0 μg/L, and the correction curve is Y=2.59×103 X-0. 057,r=0. 9992, with good linear relationship, the range of recovery was 82.0% ~ 105.5%.[Conclusion]The method is accurate, stable and sensitive. The average mass concentration of volatile phenol in marine fish is significantly higher than that in freshwater fish, and among the marine fish 25.7% is over standard, mainly in silver pomfret and Tsim Sha fish. No over-standard case was found in freshwater fish.