Flow injection analysis of volatile phenol in fish with 4-amino antipyrine colorimetry

Objective To investigate the detection of volatile phenol in fish by flow injection analysis with 4-amino antipyrine colorimetry.Methods The linear range,detection limit,recovery,and relative standard deviation of flow injection analysis for volatile phenol with 4-amino antipyrine colorimetry were tested,and 52 samples of marine fish and 42 samples of freshwater fish were detected.Results The linear range was 0.001-0.04mg/kg;the detection limit was less than 0.001 mg/kg;the recovery was 89.5%-102.9% and the relative standard deviation was 1.3-4.6%.The average concentration of volatile phenol in marine fish(0.184 mg/kg)was significantly higher than that of in freshwater fish(0.061 mg/kg)(P0.05).The volatile phenol concentration of freshwater fish was 24% higher in 2009 than that of in 2007(0.049 mg/kg).Conclusion The high automation and micro-operation improve the sensitivity and stability of flow injection analysis with 4-amino antipyrine colorimetry and the method is efficiency and energy saving.