Analysis of Genetic Diversity of Brassica napus Mainly Popularized in Hanzhong Based on SRAP and SSR Markers

【Objective】This study was to understand the genetic diversity,genetic background and relationship of all rapeseed mainly popularized in Hanzhong,and to provide the theoretical basis for rapeseed breeding.【Method】We investigated genetic diversity of 34 main rapeseed strains mainly popularized in Hanzhong by utilizing 24pairs of SRAP makers and 17pairs of SSR.【Results】Total of 145alleles were detected by using SRAP markers and 99alleles were detected by using SSR.An average of 6alleles were obtained per pair of molecular markers.The PIC for SRAPs were between 0.19and 0.74with an average of 0.48,while the PIC for SSRs were between 0.12and 0.74with the average of 0.40.Cluster analysis showed that genetic similarity coefficient of 34testing materials of rapeseed centralized in the range of 0.56to 0.94.The genetic distances were between 0.62and 0.91.【Conclusion】The SRAP possessed higher potential in determining genetic diversity when compared with SSR.The cluster analysis results obtained by SRAP were closer to pedigree method.Overall,the tested rapeseed varieties have a close genetic relationship.