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2010•Strait Pharmaceutical JournalRequires access

RP-HPLC fingerprint spectrum of Rhizoma et Radix Ligustici

Sun Qi-shi

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Abstract

OBJECTIVE To establish the chromatographic fingerprint analysis for the quality control of Rhizoma et Radix Ligustici.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.The reversed phase HPLC system consisting of a C18 column(200mm×4.6mm,5μm)and a mixture of acetonitrile(A)and 0.04% phosphoric acid (B)with gradient elution(0～15min,90%→80% B,15～60min,80%→15% B,volume fraction)as the mobile phase was adopted.The column temperature was set up at 25℃ and the flow-rate was 0.8 mL.min-1.The absorbance was monitored at 274nm.RESULTS RP-HPLC fingerprint have set up and owned the mode in common.,with better relevance.CONCLUSION The RP-HPLC fingerprint method is repeatable,feasible in analysis of Rhizoma et radix ligustici and can be used scitentifically to evaluate the quality of Rhizoma et radix ligustici.

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What this paper is about

OBJECTIVE To establish the chromatographic fingerprint analysis for the quality control of Rhizoma et Radix Ligustici.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.The reversed phase HPLC system consisting of a C18 column(200mm×4.6mm,5μm)and a mixture of acetonitrile(A)and 0.04% phosphoric acid (B)with gradient elution(0～15min,90%→80% B,15～60min,80%→15% B,volume fraction)as the mobile phase was adopted.The column temperature was set up at 25℃ and the flow-rate was 0.8 mL.min-1.The absorbance was monitored at 274nm.RESULTS RP-HPLC fingerprint have set up and owned the mode in common.,with better relevance.CONCLUSION The RP-HPLC fingerprint method is repeatable,feasible in analysis of Rhizoma et radix ligustici and can be used scitentifically to evaluate the quality of Rhizoma et radix ligustici.

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Available abstract

OBJECTIVE To establish the chromatographic fingerprint analysis for the quality control of Rhizoma et Radix Ligustici.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.The reversed phase HPLC system consisting of a C18 column(200mm×4.6mm,5μm)and a mixture of acetonitrile(A)and 0.04% phosphoric acid (B)with gradient elution(0～15min,90%→80% B,15～60min,80%→15% B,volume fraction)as the mobile phase was adopted.The column temperature was set up at 25℃ and the flow-rate was 0.8 mL.min-1.The absorbance was monitored at 274nm.RESULTS RP-HPLC fingerprint have set up and owned the mode in common.,with better relevance.CONCLUSION The RP-HPLC fingerprint method is repeatable,feasible in analysis of Rhizoma et radix ligustici and can be used scitentifically to evaluate the quality of Rhizoma et radix ligustici.

Key concepts: Chromatography, Fingerprint (computing), Radix (gastropod), Phosphoric acid, Chemistry, High-performance liquid chromatography, Gradient elution, Computer science

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