Dynamic changes of alkaloid content in seed of Sophora alopecuroides at different phenological phases

Objective To study the dynamic changes of alkaloid content in seed of Sophora alopecuroides at different phenological phases,and explore the optimal harvest period of Sophora alopecuroides L. and effective use of resources in the alkaloids industrialization production. Methods The contents of main alkaloids from the different phenological phases were analyzed by HPLC. Chromatographic column: X-Brige C18 column( 250 mm × 4. 6 mm,5μm); Mobile phase: acetonitrile( A) and 0. 05mol / L phosphoric acid solution( B),gradient elution:( 0-10 min,94% B; 10-50 min,87% B; 50-55 min,94% B); Flow rate: 1. 0mL / min; Wavelength: 205nm and 311 nm. The column temperature: 25℃. Results The contents of different alkaloids showed different variations with changes in phenological phases,which the contents of oxymatrine,oxysophocarpine and cytisine decreased from August to October; sophoridine,oxymatrine,and sophoramine increased from May to July; sophocarpine,matrine and Sophocarpine with the phenological phases did not change significantly. And the main alkaloids were oxymatrine and oxysophocarpine. Conclusion The results provide a basis for the appropriate harvest stage for the different alkaloids in industrial production.