Cloning and Sequence Analysis of Mycobacterium paratuberculosis SOD Gene

The genomic DNA was extracted from Mycobacterium paratuberculosis C2,the mature secreted protein SOD gene was amplified with a pair of specific primers through using polymerase chain reaction(PCR).The PCR product was approximately 620 bp DNA segment.The PCR product was cloned into pGEM-T vector,then by using α-complementation test,restrictional enzyme assay and recombinant plasmid sequence analysis,Recombinant plasmid pGEM-T-SOD was successfully constructed.These results could serve as a basis for further studies on the usefulness of SOD gene and immunogenicity of SOD gene expression product.