Cloning and Sequential Analysis of Mycobacterium bovis RecA Gene

The chromosome DNA was extracted from Mycobacterium bovis Vallee111,the RecA gene was amplified with a pair of specific primers by using polymerase chain reaction(PCR).The PCR product was approximately 240 bp DNA segment.The PCR product was cloned into pMDTM18-T Vector,then by using α-complementation test,PCR assay and recombinant plasmid sequential analysis,recombinant plasmid pMDTM18-T-RecA was successfully constructed.These results could serve as a solid basis for further studies on the usefulness of RecA gene and its application in diagnosis of tuberculosis.