Rapid Propagation Technique of Cutting Rose

The clones of three kinds of cutting rose were constructed by tissue culture to explore the suitable culture medium for rapid propagation of cutting rose and provide a theoretical basis for mass propagation of cutting rose seedlings.The results showed that the preferable media for producing buds was 1/2 MS+6-BA 1.5 mg/L+NAA 0.5 mg for kalola(budding rate 81%),MS+6-BA 0.8 mg/L+NAA 0.1 mg/L for pink(budding rate 79%) and MS+6-BA 1.0 mg/L+NAA 0.1 mg/L for snow(budding rate 75%).The optimum multiplication medium for kalola,pink and snow were 1/2 MS+6-BA 1.0 mg/L+NAA 0.3 mg/L(multiplication coefficient 4.67),MS+6-BA 0.5 mg/L+NAA 0.1 mg/L(multiplication coefficient 3.15) and MS+6-BA 1.0 mg/L+NAA 0.05 mg/L(multiplication coefficient 3.33) respectively.1/2 MS+NAA 0.5 mg/L+IBA 0.1 mg/L was the optimum rooting medium for kalola;MS+NAA 0.1 mg/L+IBA 0.1 mg/L was the optimum rooting medium for pink;1/2 MS +NAA 0.5 mg/L was the optimum rooting medium for snow.Transplanting survival rate was all obviously high with vermiculite as media.