Study on Tissue Culture and Rapid Propagation of Wild Lilium papilliferum

[Objective] To screen out the optimal medium for tissue culture of wild Lilium papilliferum.[Method]The tissue culture of wild Lilium papilliferum was studied by using its scales,bulblet,stem pieces and leaves as explants respectively.The basic mediums were MS,B5 and White that included 0.03 mg/L of NAA.Four differential mediums were: MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L 6-BA+1.00 mg/LIAA,MS+0.10 mg/L KT+0.10 mg/NAA and MS+0.03 mg/LNAA.Six proliferation formulas were as below: MS+0.03 mg/L NAA,MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10 mg/L KT,MS+0.20 mg/L KT+0.20 mg/L NAA and MS+0.10 mg/L KT+0.15 mg/L NAA.Four rooting media were: MS+1.00 mg/L IBA,MS+1.00 mg/L IBA+0.20 mg/L 6-BA,1/2MS+1.00 mg/L IBA+0.20 mg/L 6-BA and White+1.00 mg/L IBA+0.20 mg/L 6-BA.[Result] MS medium was the optimum basic medium.The optimal differential mediums for scale,bulblet,leaf and stem pieces of wild Lilium papilliferum were MS+0.03 mg/L NAA,MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L IAA,and MS+2.00 mg/L 6-BA+0.20 mg/L NAA,respectively.The optimum subculture medium was MS+ 0.10 mg/L KT +0.15 mg/L NAA and the optimal medium for rooting of rootless bulbs and seedlings was 1/2MS+0.20 mg/L 6-BA+1.00 mg/L IBA.In the field,the tissue culture seedlings formed by scales and bulblets grew more strongly than others.[Conclusion] The optimal tissue culture mediums for wild Lilium papilliferum were obtained in this work,which provided basis for the rapid propagation of wild Lilium papilliferum.