Cloning and bioinformatics analysis of the structure protein gene of bovine viral diarrhea virus strain yak

The structure protein research and analysis of the BVDV separated from the yak body for the first time is of great theoretical value and academic significance.According to the sequence data of BVDV strain published by GenBank,four sets of primers are designed and used to amplify structure protein gene of BVDV strain yak by the method of PCR.A specific 2700bp DNA segment is amplified,which is cloned into pMD19-T Vector.The positive recombinant clone is identified by plasmid PCR and restriction enzyme digestion.The recombinant plasmid is sequenced.Compared with DNAMAN,the structure protein gene of BVDV strain yak exhibits the highest homology with strain NADL,but they only share 70.1% nucleotide sequence identity and 74.4 % amino acid identity,showing that the structure protein gene of BVDV strain yak has great variation.Bioinformatics analysis shows that the structure of sequence fragments of high water,protein gene amino acid sequence with nucleic is hydrophilic into parallel related,hydrophilic and nucleic standard strain very similar.