Cloning and Bioinformatics Analysis of the P125 Gene of Bovine Viral Diarrhea Virus Strain Yak

According to the sequence data of BVDV strain published by GenBank,five set of primers were designed and used to amplify P125 gene of BVDV strain yak by the method of PCR.A specific 3475 bp DNA segment was amplified,which was cloned into pMD19-T vector.The positive recombinant clone was identified by plasmid PCR and restriction enzyme digestion.Compared with BLAST,the P125 gene of BVDV strain yak exhibited the highest homology with strain NADL,but they only shared 63.4%,showing that the P125 gene of BVDV strain yak had great variation.This might be the virus to adapt to yak and the ecological environment of the plateau,or the virus might also have an independent source of genetic.