Effect of the Different Medium on the Growth of Potato Plantlet in Vitro
Liu Dao-min
Abstract
Liu Dao-min
Abstract
The purpose of the study was to investigate the effect of different medium on the growth of potato plantlets in vitro and get the optimum medium.The stems of Zhongshu 4 were cultured with different medium by agar solid culture.The growth of plantlets in vitro was observed and their root number,root length,stem length,stem diameter,leaf number and yield were measured.There was no significant difference in the potato plantlets in vitro in different medium in the initial growth stage,but their anaphase of growth stage was different.The growth of plantlets in vitro in the medium of potato culture medium + Mn 0.16 mg/L + Mo 0.1mg/L +1/4 MS(NH4NO3+KNO3+KH2PO4)was best among 6 kinds of media.The seedlings were strong,orderly,uniform and the area of leaf was large,with the cultivation survive rate in protected land was 100%,with the highest yield.The study supplied a theoretical basis for optimizing the medium for the growth of potato plantlet in vitro.
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The purpose of the study was to investigate the effect of different medium on the growth of potato plantlets in vitro and get the optimum medium.The stems of Zhongshu 4 were cultured with different medium by agar solid culture.The growth of plantlets in vitro was observed and their root number,root length,stem length,stem diameter,leaf number and yield were measured.There was no significant difference in the potato plantlets in vitro in different medium in the initial growth stage,but their anaphase of growth stage was different.The growth of plantlets in vitro in the medium of potato culture medium + Mn 0.16 mg/L + Mo 0.1mg/L +1/4 MS(NH4NO3+KNO3+KH2PO4)was best among 6 kinds of media.The seedlings were strong,orderly,uniform and the area of leaf was large,with the cultivation survive rate in protected land was 100%,with the highest yield.The study supplied a theoretical basis for optimizing the medium for the growth of potato plantlet in vitro.
Key concepts: Plantlet, In vitro, Agar, Murashige and Skoog medium, Horticulture, Biology, Yield (engineering), Botany