Effects of Ulinastatin on the Expressions of IL-8 and TNF-αin Rats with Severe Acute Pancreatitis

Objective To study the effects of ulinastatin on the expressions of IL-8 and TNF-α in rats with severe acute pancreatitis.Methods Ninety healthy male SD rats were divided into three groups: blank control group(30 rats),SAP group(30 rats) and ulinastatin treatment group(30 rats).Each group was divided into three subgroups: 6h group,12h group and 24h group.SAP model was established by injecting 5% sodiumtauro cholate via retrograde bile and pancreaticduct.The treatment groups were injected with ulinastatin via hepatic portal vein.The blank control group and SAP group was injected with physiological saline.Pathological changes were performed with the aid of hematoxylin-eosin staining.Detect the changes of serum amylase,IL-8 and TNF-α in different groups.Results Compared to the SAP group,the rats in ulinastatin treatment group had a lower pathological damage,the level of serum amylase,IL-8 and TNF-αwere significantly decreased in ulinastatin treatment groups(P0.05).Conclusion Ulinastatin can ameliorate the pathological damage in rats of SAP.The effect of ulinastatin on SAP may be related to decreasing the expressions of IL-8 and TNF-α.