Screening and Cloning of Genes Related to Uterine Leiomyoma

[Objective] To study the alterations of gene expression between uterine leiomyoma tissues and normal myometrium uterine tissues; to screen and clone the genes related to uterine leiomyoma. [Methods] To compare different genes expressed in 11 uterine leiomyoma tissues and normal myometrium uterine tissues by fluorescent differential display reverse transcription polymerase chain reaction (FDD-RTPCR). Differentially expressed cDNA fragments were cloned, sequenced, and analyzed in origin. And five of differentially expressed bands were analyzed by RT-PCR. [Results] The expression of NO. 3, 5, 11, and 12 cDNA fragments in uterine leiomyoma were differential. NO. 3 cDNA fragment was expressed in eight of uterine leiomyoma tissue (8/10), but in two of corresponding normal myometrium tissue. NO. 5, 11, and 12 cDNA fragments were expressed higher in uterine leiomyoma tissue than matched myometrium. [Conclusion] During occurrence and development of uterine leiomyoma, expression of more than one gene altered in uterine myometrium, ulap8 gene may be expressed specially in uterine leiomyoma tissue. ulap14, ulap25, and ulap26 genes were expressed higher in uterine leiomyoma tissue than matched myometrium, it may be related to the development of uterine leiomyoma.