2008•Journal of Cardiovascular and Pulmonary DiseasesRequires access
Isolation and inducement of endothelial progenitor cells from rabbit bone
Guan Hen
Open publisher page 0 citations
Abstract
Objective:To investigate the method of isolation and inducement of endothelial progenitor cells from rabbit bone marrow.Method:Rabbit bone marrow was obtained by iliac crest puncture, then bone marrow mononuclearcells were isolated by gradient centrifugation using Ficoll isolative liquid. Mononuclearcells were then cultivated with M199 culture medium and induced to EPCs by VEGF, bFGF and IGF-1.Cultivated cells were identified by immunofluorescence and electron microscope.Result:Attached cells appeared after 48-72 hours culture and become fusiform shape gradually. Attached cells formed several cord-like structure after 8 days culture, which is the character of endothelial cells. Cell clusters were observed after 7-10 days from which attached cells were budding. Cells mixed together after 14 days and were proved to be EPCs by electron microscope and Immunohistochemistry results.Conclusion:The mononuclear cells isolated form rabbit bone marrow by gradient centrifugation can be induced into endothelial progenitor cells in vitro.