Protective effects of aspirin on kidney and liver in rats poisoned by paraquat

Objective To investigate the protective effect of aspirin on kidney and liver in the rats poisoned by paraquat (PQ).Methods Sixty healthy Sprague-Drawley (SD) rats were randomly divided into PQ exposure group,aspirin-DL-lysine treatment group and 0.9% NaCl control group,20 rats in each group.Rats in PQ exposure group were given PQ (50 mg/kg) o-rally,and 0.9% NaCl saline (1 ml) via intraperitioneal injection 3 h later.Rats in aspirin-DL-lysine treatment group were given PQ (50 mg/kg) orally,and an intraperitioneal injection with As-pirin-DL-lysine (200 mg/kg) 3 h later.Rats in control group were given0.9% NaCl saline (2 ml) orally,and an injection of 1 ml 0.9% NaCl intraperitioneally 3 h later.Rats were sacrificed to obtain blood from abdominal aorta on the 1st,3rd,7th,and 14th d.Then the levels of alanine transaminase (ALT),aspartate aminotransferase (AST),creatinine (Cr),and blood urea nitrogen (BUN) were detected by automatic biochemistry analyzer and pathological changes of liver and kidney were observed.Results Compared with the treatment and control groups,the levels of ALT,AST,Cr,and BUN in the PQ exposure group increased obviously on the 3rd,7th and 14th d (P0.05),and the levels of them in the treatment group were also higher than the control group on the 1st,3rd,and 7th d (P0.05).Compared with the control group,the levels of ALT,AST,Cr,and BUN increased obviously in the PQ exposure group (P0.05),but there was no statistical difference between the PQ exposure group and the treatment group on the 1st d (P0.05).On the 14th d,no significant differences were found between the treatment and control groups (P0.05).The main pathological changes in the PQ exposure group were congestion,edema,inflammatory cell infiltration,vacuolar degeneration,necrosis and disorganization,while in the treatment group the changes were slighter without disorganization.Conclusion Asipirin may protect the kidney and liver of rats poisoned by paraquat.