EXOGENOUS 5'-NECLEOTIDES PROMOTE RESTORATION AFTER LIVER DAMAGE INDUCED BY ALCOHOL IN RATS

Objective To investigate the effects of exogenous 5'-nucleotides supplemented diets on early alcoholic liver disease in Wistar rats.Method To build alcoholic liver disease model,48 male Wistar rats were gavaged with 50% ethanol and 12 rats with distilled water as control.After 4 w,11 model rats died.Eight of the model rats and all control rats were killed to observe whether the model was established.Blood and hepatic tissue of the rats were collected.The level of alanine amino transferase(ALT),aspartate amino transferase(AST),triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),total protein(TP),albumin(ALB),the activities of superoxide dismutase(SOD),and the content of malondialdehyde(MDA) in plasma were measured.Liver histology was assessed.In the later 2 w,the left 29 model rats were divided into 3 groups,9 rats were fed diets without nucleotides(MN),10 in each,with low and high dose of 5'-nucleotides(ML and MH) supplemented diets.Results Compared with controls,the model group had higher hepatosomatic index,higher plasma ALT,AST,TC,HDL-C level and liver histological study showed mild injury.The changes recovered 2w after alcohol intake.Furthermore,plasma TG was lower and SOD activities was higher in ML group,while plasma TG was lower and ALB,albumin/globulin,and SOD activities was higher in MI group than MN group.Conclusion Exogenous nucleotides from diet could promote restoration of alcoholic liver injury in rats.