Effect of S-nitrosoglutahione on vascular smooth muscle cell proliferation induced by endothelin

Objective To investigate the effect of S-nitrosoglutahione(GSNO) on SD rat vascular smooth muscle cells(VSMCs) proliferation induced by endothelin(ET). Methods In vitro, the rat thoracic aortic VSMCs were cultured and induced by ET, and then treated with different dose of GSNO. The change of VSMCs proliferation was determined by MTT and BrdU-ELISA incorporation methods. The proliferation cycle of VSMCs was observed by flow cytometric analysis. Results ET significantly accelerated DNA synthesis and the VSMCs proliferation. Compared with ET group,the A value and BrdU-ELISA incorporation were significantly reduced in the GSNO groups with different dose(P0.05). The percentage of cells in DNA synthetic( S ) and mitotic phase ( G2/M ) groups was lower than that of ET group(P005). With the increasing dose of GSNO, the inhibition became more significant.Conclusion To certain extent,GSNO could inhibit the proliferation of VSMCs induced by ET in dose-dependence manner.