Study on the extraction process of polysaccharides from Fenugreek

Objective To optimize the extraction process for the polysaccharides from Fenugreek. Methods The orthogonal design was used to evaluate the effects of extracting time,times and solid-liquid ratio with the yields of dry extract and polysaccharides as indexes. The phenol-sulfuric acid method was adopted to determine the polysaccharides. Results The results showed that the order of the selected factors influencing the extraction polysaccharides of fenugreek were as follows:extraction times soild-liquid extraction time. In the optional extraction process,the liquid-soild ration was 20∶ 1,extracting for 3 times and 1. 5 h each time. Conclusion The extraction process was reasonable and feasible,which was suitable for the extraction of polysaccharides from Fenugreek.