Studies on Qualitative and Quantitative Methods of Shenbai Concentrated Pills

Objective:To study the qualitative and quantitative methods of Shenbai concentrated pills.Methods: Danshen root and lightyellow sophora root in Shenbai concentrated pills were identified with the method of TLC.The contents of astragaloside Ⅳ and paeoniflorin were determined with the method of HPLC.Results: For TLC,the spots of the two herbal drugs were well separated and without interference.Quantitative analysis of HPLC showed that the average recovery of astragaloside Ⅳ was 100.82%(RSD1.73%) with the RSD values of precision,repeatability and stability tests were 2.79%,1.48% and 1.44%,respectively;and the average recovery of paeoniflorin was 99.83%(RSD2.34%) with the RSD values of precision,repeataibility and stability tests were 2.25%,2.16% and 1.60%,respectively.Conclusions: The methods set up by this study are accurate and easy to perform with the merits of good resolution,specificity and reproducibility.It could effectively control the quality of Shenbai concentrated pills.