2012•Kunming Yike Daxue xuebaoRequires access

Study on the HPLC Fingerprints of Radix puerariae in Yunnan Province

Leng Hong-qiong

Abstract

Objective To establish the simple and feasible analysis method of fingerprint for Radix puerariae by HPLC.Methods The fingerprint was investigated using waters C18(4.6 mm 250 mm,5 μm).The mobile phase consisted of acetonitrile 0.4% glacial acetic acid and eluted in gradient mode.The flow rate was 1.0 mL/min.The detection wavelength was 250 nm.Result The common mode of HPLC fingerprint was developed and quality of Radix puerariae in Yunnan province was similar.Conclusion The method is simple and accurate with good reproducibility,which can be used for indentify and quality control of Radix puerariae.

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Available abstract

Key concepts: Chromatography, Radix (gastropod), Fingerprint (computing), Gradient elution, Acetic acid, High-performance liquid chromatography, Reproducibility, Chemistry