EFFECTS OF DIFFERENT CULTURAL CONDITIONS ON CALLUS GROWTH AND FLAVONOID YIELD OF Carpesum.macrocephalum

The objective of the current study was to set a foundation for efficient large scale cultivation for biosynthesis of flavonoids.The effect of several factors on callus growth amount,flavonoid content and yield in callus cultures of Carpesium.macrocephalum Franch.et Sav were investigated.The results showed that: ① the flavonoid content and yield of root-induced callus was higher than hypocotyl and cotyledon-induced callus;② the flavonoid yield of root-induced callus was stable during 3～12 subculture generation;③ the optimum temperature for callus growth and flavonoid cumulation was 25℃;④ the optimum ultraviolet radiation time for callus flavonoid content was 10 min;⑤ the callus growth and flavonoid yield increased first and then decreased at different culture medium,the flavonoid yield reached the maximum after 35～40 days of inoculation,and the optimal culture medium to produce flavonoid was NT.