Segmental Distribution and Ontogenetic Regulation of NHE3 mRNA Expression in Intestine of Pigs

Segmental distribution and ontogenetic regulation of sodium hydrogen exchanger(NHE3) mRNA expression were evaluated in pigs along the horizontal axis of the intestine.A total of 35 littermate purebred Lantang gilts and 35 littermate purebred Landrace gilts were divided into seven groups at the ages of day 1,7,26,30,60,90 and 150,respectively.Samples of intestinal segments(duodenum,jejunum,ileum and colon) were collected.The NHE3 mRNA abundance was determined by real-time RT-PCR using SYBR GreenⅠRT-PCR mix Kit.Results showed that the abundance of NHE3 mRNA was highest in colon and lowest in jejunum and ileum(P0.05).The ontogenetic expression of NHE3 mRNA in duodenum and jejunum of Lantang and Landrace pigs were similar.The highest expression abundance of NHE3 mRNA were 7 d of Lantang and 30 d of Landrace in duodenum,7 d of Lantang and 26 d of Landrace in jejunum(P0.05).The expression pattern of NHE3 mRNA in colon was differed from duodenum and jejunum.Abundance of NHE3 mRNA of Lantang pigs on 26,90 and 150 d were lower than that of Landrace pigs(P0.05) in colon.The results indicated that NHE3 mRNA expression could be regulated by developmental stages,breeds and segments of intestine in pigs.