2009•Journal of the South China Agricultural UniversityRequires access
Regional and ontogenetic expression of CDX2 mRNA in intestine of chicken.
Xun Zhan, You Feng, Xiuqi Wang, Gang Shu, Qingyan Jiang, Feng DingYuan, Xugang Shu
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Abstract
One hundred and twenty 1-day-old parental male Arbor Acre(AA) broiler chicks were randomly divided into 4 replicates,respectively.Regional expression of CDX2 mRNA in different segments of intestinal tract of AA chicken on 30 d and ontogenetic expression of CDX2 mRNA in duodenum and jejunum of AA broiler chickens were determined by relative quantitative RT-PCR.It was found that: The CDX2 mRNA expression in duodenum was higher than that in jejunum(P=0.09),ileum(P=0.06) and colorectum(P=0.05),respectively.Ontogenetic patterns of CDX2 mRNA were consistent between duodenum and jejunum.The abundance of CDX2 mRNA decreased from day 2 to day 30,increased from day 30 to day 44 and increased slightly on day 58.The abundance of CDX2 mRNA on day 2 and day 44 were higher than on day 30(P0.05),respectively.These results indicate that: Regional expression of CDX2 mRNA in intestine of AA chickens was declined along the downward intestine from proximal intestine to distal intestine(P0.05).Ontogeny of CDX2 mRNA in AA chickens had the same pattern in duodenum and jejunum,indicating that CDX2 mRNA expression could be regulated by developmental stage.