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Study on Bioactive Peptide Preparation from Egg White by Neutral Protease Hydrolysis

Jin Lei

Abstract

Bioactive peptide was prepared by AS1398 neutral protease hydrolysis from egg white.The optimum parameters were 8.0 pH,60 ℃ reaction temperature,4 % substrate concentration,7 % enzyme concentration of sabstrate for hydrolysis 4 h.Under this condition the hydrolysis degree reached 26.1 %.Molecular weight of peptide was determined by Sephadex G-15.The result showed that molecular weight of most peptides was about 1300Da.

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Key concepts: Hydrolysis, Sephadex, Protease, Chemistry, Peptide, Egg white, Enzymatic hydrolysis, Chromatography