Effect of Estrogen or Progesterone Treatment on Mast Cell Number in the Uterus of Ovariectomized Rat

To discuss the relationship among mast cells,estrogen and progesterone in uterus,the effect of estrogen or progesterone on the number of mast cells in the uterus of ovariectomized rats were studied.35 female rats were devided into 7 groups(SHAM,ovariectomized(OVX),OVX+E 20 μg,OVX+E 100 μg,OVX+E 500 μg,OVX+P 2 mg,OVX+P 10 mg).The concentration of estradiol and progesterone in serum were detected by RIA,and the uterine mast cells were stained by toluidine blue.The results were as follows:①Compared to ovariectomized rat,the concentration of estradiol in serum increased by 97.13% in OVX+E 20 μg(P0.05),204.84% in OVX+E 100 μg(P0.05) and 936.45% in OVX+E 500 μg group(P0.05);The progesterone concentration increased by 77.25% in OVX+P 2 mg(P0.05) and 235.25% in OVX+P 10 mg group(P0.05).②Compared to ovariectomized rat,the number of mast cells in uteri decreased by 32.65% in OVX+E 20 μg,64.50% in OVX+E 100 μg(P0.05),74.49% in OVX+E 500 μg(P0.05) and 70.67% in OVX+P 10 mg groups(P0.05).However,the number of mast cells increased by 66.73% in OVX+P 2 mg group(P0.05) compared with OVX.The trend of mast cells number in the rat uterus was decreased gradually with the increasing of estrogen or progesterone concentration.The number of mast cells in ovariectomized rat uterus was affected by estrogen or progesterone.These results demonstrated that estrogen or progesterone directly affected the number of mast cells in the uterus of rat.