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Effect of estrogen and progesterone on the expression of 1, 25-dihydroxyvitamin D receptors mRNA in the liver of ovariectomized rats.

Yun Zhou, Ren-qing Ye, Dehong Cai, Hua Zhang

Open publisher page 6 citations

Abstract

OBJECTIVE: To study the effect of estrogen and progesterone on the expression of dihydroxyvitamin D receptor (VDR) mRNA in the liver of ovariectomized rats. METHODS: Twenty-five adult female SD rats were randomly divided, with equal numbers, into sham-operated group (sham), ovariectomized group (OVX), ovariectomized group with estrogen treatment (OVX+E), ovariectomized group with progesterone treatment (OVX+P) and ovariectomized group with both estrogen and progesterone treatment (OVX+E+P). After 3 months and a half of feeding, all animals were killed to assess VDR mRNA by way of reverse transcriptase-PCR (RT-PCR). RESULTS: RT-PCR revealed marked increase in the band intensity corresponding to VDR mRNA product in Sham, OVX+E, and OVX+E+P groups. CONCLUSION: Estrogen may increase the transcription level of VDR gene in the liver of ovariectomized rats.

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OBJECTIVE: To study the effect of estrogen and progesterone on the expression of dihydroxyvitamin D receptor (VDR) mRNA in the liver of ovariectomized rats. METHODS: Twenty-five adult female SD rats were randomly divided, with equal numbers, into sham-operated group (sham), ovariectomized group (OVX), ovariectomized group with estrogen treatment (OVX+E), ovariectomized group with progesterone treatment (OVX+P) and ovariectomized group with both estrogen and progesterone treatment (OVX+E+P). After 3 months and a half of feeding, all animals were killed to assess VDR mRNA by way of reverse transcriptase-PCR (RT-PCR). RESULTS: RT-PCR revealed marked increase in the band intensity corresponding to VDR mRNA product in Sham, OVX+E, and OVX+E+P groups. CONCLUSION: Estrogen may increase the transcription level of VDR gene in the liver of ovariectomized rats.

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Available abstract

OBJECTIVE: To study the effect of estrogen and progesterone on the expression of dihydroxyvitamin D receptor (VDR) mRNA in the liver of ovariectomized rats. METHODS: Twenty-five adult female SD rats were randomly divided, with equal numbers, into sham-operated group (sham), ovariectomized group (OVX), ovariectomized group with estrogen treatment (OVX+E), ovariectomized group with progesterone treatment (OVX+P) and ovariectomized group with both estrogen and progesterone treatment (OVX+E+P). After 3 months and a half of feeding, all animals were killed to assess VDR mRNA by way of reverse transcriptase-PCR (RT-PCR). RESULTS: RT-PCR revealed marked increase in the band intensity corresponding to VDR mRNA product in Sham, OVX+E, and OVX+E+P groups. CONCLUSION: Estrogen may increase the transcription level of VDR gene in the liver of ovariectomized rats.

Key concepts: Ovariectomized rat, Internal medicine, Endocrinology, Estrogen, Estrogen receptor, Calcitriol receptor, Messenger RNA, Estrogen receptor alpha

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Effect of estrogen and progesterone on the expression of 1, 25-dihydroxyvitamin D receptors mRNA in the liver of ovariectomized rats. — Research Paper | ScholarLens