2014•Kunming Yike Daxue xuebaoRequires access
Expressions of HO-1 in the Lung of Paraquat Poisoning Rats
Na Wang
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Abstract
Objective To observe the histological changes of lung tissues after poisoned by paraquat,and explore the expressions of hemeoxygenase-1(HO-1) in lung of paraquat poisoning rats.Methods One hundred and fifty SD male rats were randomly divided into 3 groups: normal control group(C) 30 rats,paraquat poisoning group(PQ) 60 rats and drug intervention group(PNS) 60 rats. The PQ and PNS group rats were given paraquat by gastric perfusion(25 mg/kg);The C group rats were given saline by gastric perfusion.The rats in PNS group were protected by intravenous injections of penis with PNS(50 mg/kg) 15 min before exposure of paraquat,once a day until killed. The PQ and C group rats were given saline. Histological changes of lung tissues under light microscope were observed.The expression of HO-1mRNA in lung tissues of rats were measured by RT-PCR and The HO-1protein was evaluated by western blot at different time of 6 h.12 h.1 d.3 d and 7d after poisoning.Results The degree of lung injury in PQ group was higher than that in C group. But in PNS group the degree of lung injury was lighter. The relative magnitude expression of HO-1 mRNA and HO-1 protein in group C were weak or no. The relative magnitude expression of HO-1 mRNA and HO-1 protein in PNS(6 h), PNS(12h) PNS(1d) and PNS(3d) subgroups were significantly higher as compared with the PQ group(P0.05).Conclusions PNS can effectively improve the expression of HO-1 protein and mRNA in lung tissue and reduce the pathological changes of lung tissue in rats with paraquat poisoning.