The protective effect and mechanism of S-ademetionine on hepatic partial ischemia-reperfusion injury in rats

Objective: To investigate the protective effect and mechanism of S-ademetionine against hepatic ischemia-reperfusion injury in rats and its mechanism. Methods: Thirty-six SD female rats were randomly divided into 3 groups, SHAM:sham operation group, IR: ischemia-reperfusion group, SAMe: S-ademetionine treatment group. The model of 70% hepatic ischemia-reperfusion was established in the rats. Ischemia lasted for 1 h. The rats of SAMe group received SAM(100 mg/kg)intraperitoneally 1 d prior to operation and 1 h after operation. The other groups received saline solution at the same time.Rats were killed at 6,24 h after reperfusion respectively. Blood samples were collected and analyzed for the levels of serum,alanine aminotransferase(ALT), aspertate aminotransferase(AST), tumor necrosis factor α(TNF-α). The malonyldialdehyde(MDA) in liver tissues were measured and histologic changes were observed in HE staining. Results: Comparison to IR group,SAMe group was associated with lower serum transaminases ALT, AST, TNF-α. Besides, MDA expression in SAMe group was significantly lower than those in IR group. The injury of liver tissue in IR group was more obviously. Conclusion: S-ademetionine has a protective effect in hepatic ischemia-reperfusion injury in rat. The mechanism of protection appears to be dependent on its inhibitory effect on neutrophil infiltration and inflammatory cytokines.