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2014•Zhejiang linye kejiRequires access

Experiment on Inhibitory Effect of TanshinoneIAgainst Mouse B16 Melanoma Cells

Le Zhang

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Abstract

Experiments were conducted on effect of different concentrations of TanshinoneⅠ(90%) against in vitro cultured mouse B16 melanoma cells. Cell growth inhibition rate was determined by MTT method. Treated cells were observed, collected and stained by PI. Cell cycles and apoptosis rate were detected by flow cytometry(FCM). Results demonstrated that different concentration(5, 4, 3 μg /ml) of Tanshinone Ⅰ had evident inhibitory effect on B16 melanoma cells with dosage and duration, and the composition of B16 cell cycles changed.

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What this paper is about

Experiments were conducted on effect of different concentrations of TanshinoneⅠ(90%) against in vitro cultured mouse B16 melanoma cells. Cell growth inhibition rate was determined by MTT method. Treated cells were observed, collected and stained by PI. Cell cycles and apoptosis rate were detected by flow cytometry(FCM). Results demonstrated that different concentration(5, 4, 3 μg /ml) of Tanshinone Ⅰ had evident inhibitory effect on B16 melanoma cells with dosage and duration, and the composition of B16 cell cycles changed.

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Available abstract

Experiments were conducted on effect of different concentrations of TanshinoneⅠ(90%) against in vitro cultured mouse B16 melanoma cells. Cell growth inhibition rate was determined by MTT method. Treated cells were observed, collected and stained by PI. Cell cycles and apoptosis rate were detected by flow cytometry(FCM). Results demonstrated that different concentration(5, 4, 3 μg /ml) of Tanshinone Ⅰ had evident inhibitory effect on B16 melanoma cells with dosage and duration, and the composition of B16 cell cycles changed.

Key concepts: Melanoma, Flow cytometry, Inhibitory postsynaptic potential, Apoptosis, In vitro, Cell, Molecular biology, MTT assay

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