2009•Journal of Clinical CardiologyRequires access

Nitric oxide inhibits osteopontin expression in rat aortic vascular smooth muscle cells

Tao Hu

Abstract

Objective:To investigate the effect of nitric oxide (NO) on osteopontin expression in rat aortic vascular smooth muscle cell(VSMC).Method:Rat aortic VSMC were cultured in vitro and incubated with different concentration of S-nitroso-N-acetylpenicillamine(SNAP),a nitric oxide donor for 48 hours. Then RT-PCR and Western bloting analysis were used to observe the osteopontin expression in rat VSMC.Result:Different concentration of SNAP could obviously reduce the mRNA expression and protein synthesis of OPN with a dose-dependence(P0.05). Conclusion:These results demonstrate that NO could inhibit osteopontin expression in rat VSMC.

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Key concepts: Osteopontin, Snap, Nitric oxide, Vascular smooth muscle, In vitro, Medicine, Cell, Messenger RNA