Effect of Ginsenoside Rg1 on Bcl-2 Expression in Rat Brain Slices Model of AD

Objective The purpose of this study was to investigate the effect of ginsenoside Rg1 on the Bcl-2expression in rat brain slices model of AD induced by okadaic acid(OA). Methods The brain slices of 5 week old SD rats were randomly divided into control group, model group,and ginsenoside Rg1 groups(including small,middle and large dose groups), each group of 10 brain slices. Firstly, each brain slice was put in artificial cerebrospinal fluid without any treatment to restore 1 h. Then, Rg1 was added in the small, middle and large dose groups to make the Rg1 final concentration of 60,120 and 240 μmol/L for 2 h, respectively. Lastly, OA was added in the model group and ginsenoside R1 groups to make the final concentration of 1 μmol/L for 3 h. While the blank control group was not given any treatment. After intervention, the expression of Bcl-2 protein in the brain slices was detected using the Western blot technology. Results Compared with the control group, the expression levels of Bcl-2 in the brain slices of model group was decreased significantly(P0.01). Compared with the model group,the expression levels of Bcl-2 in the three ginsenoside Rg1 groups were significantly increased(P0.01),of which the Bcl-2 expression levels in the large dose ginsenoside Rg1 group were the highest. Conclusion Ginsenoside Rg1 could inhibit neuronal apoptosis and have the neuroprotective effect on AD by up-regulating the Bcl-2 gene expression.