Interspecific hybridization and F1 hybrids identification of Chrysanthemum morifolium

Objective Interspecific hybridization was made between Chrysanthemum morifolium ‘Hongxinju’(♀),C.morifolium ‘Xinbaiju’(♀) and C.indicum(♂),C.nankingense(♂),and F1 hybrids were identificated in order to discover inheritance of main characters in Chrysanthemum morifolium.Methods Interspecific hybridization of C.morifolium were made by bud pollination.Morphological,cytological,and RAPD analyses were used for the identification of interspecific hybrids.Results The hybrids showed interspecific morphological traits of their parents or unique morphological traits their parents did not possess.RAPD results indicated that the hybrids produced specific bands of male parent or new bands absent in both parents.Chromosome number of F1 hybrids was the average of their parents.Conclusion Bud pollination could improve heterozggosity of interspecific hybridization of C.morifolium ‘Hongxinju’ and ‘Xinbaiju’.Hybrids could be aneuploid chromosomes and show the significant segregation of character.