Influence of DC-exosomes vaccine combined with cisplatin on proliferation and apoptosis in human glioblastoma U251 cells

Objective To observe the effect of cisplatin combinated with the therapeutic DC-exosomes vaccine on U251 glioma cells proliferation and apoptosis.Methods The U251 cells were divided into four groups randomly.Cells in group A were cultured with conventional nutrient solution,activated lymphocyte by DC-exosomes(2×106/mL)0.5 mL to B,cisplatin(1 mg/mL) 0.01 mL to C,activated lymphocyte by DC-exosomes(2×106/mL)0.5 mL and cisplatin(1 mg/mL) 0.01 mL to D,and train those cells 2 d.The proliferation rate of U251 were detected by MTT and the apoptosis by the flow cytometry.Result After cultured with MTT for 12,24,36,48,60 h,the OD of goroup A was respectively 1.38±0.01,1.36±0.02,1.34±0.03,1.32±0.05,1.30±0.03;the OD of goroup B was respectively 1.30±0.02,1.10±0.03,0.90±0.04,0.75±0.03,0.62±0.03;the OD of goroup C was respectively 1.00±0.04,0.80±0.02,0.55±0.04,0.30±0.03,0.15±0.04,while the OD of goroup D was respectively 0.75±0.04,0.50±0.04,0.38±0.02,0.21±0.02,0.05±0.03.The OD of group A were higher than that in group B,C,D(all P0.05),the OD of group B and C were higher than thatin group D(all P0.05).After the training of 24 h,the cell apoptosis rate of group A,B,C,D were 0.5%±0.3%,18.6%±1.7%,36.9%±4.3%,57.5%±2.1% respectively.The cell apoptosis rate of group A were higher than that in group B,C,D(all P0.05),the cell apoptosis rate of of group B and C were higher than thatin group D(all P0.05).Conclusion DC-exosomes vaccine combined with cisplatin can restrain the proliferation of U251 glioma and also lead to the apoptosis of U251 cells.