Study on Extraction Technology of Chestnut Polysaccharides

Polysaccharides were extracted from chestnut using hot water extraction and ultrasound-assisted extraction.The contents of chestnut polysaccharides extracted by the two techniques were determined by anthrone-H2SO4 method after ethanol precipitation,Sevage deproteinization and organic solvent degreasing.The optimal extraction conditions were obtained by single factor and orthogonal experiments.The results showed that,the optimal conditions of hot water extraction were: chestnut 1 g,extraction temperature 60 ℃,liquid-solid ratio 20∶1(mL∶g),extraction time 1.5 h,the yield of polysaccharides was 9.85 %;The optimal conditions of ultrasonic extraction were: extraction temperature 65 ℃,liquid-solid ratio 35∶1(mL∶g),extraction time 45 min,ultrasonic power 160 W,the yield of polysaccharides was 10.67 %.