2003•Zhongguo renshougonghuanbing zazhiRequires access
Effects on liver granuloma in mice immunized with rSj14-3-3 and rSj14-3-3/SjGST after experimental infection with Schistosoma japonicum
Luzhong Fa, Zernov Ng, Ji-Long Shen
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Abstract
Aim To observe the anti-granuloma effects of recombinant Sj14-3-3 and Sj14-3-3/SjGST against Schistosoma japonicum.Methods Female BALB/c mice were immunized with rSj14-3-3 and rSj14-3-3/SjGST.Five days after final injection,mice of each group were challenged with 40±1 S.japonicum cercariae.All the mice were killed in the 6th week after challenge so as to calculate the density of egg node in the surface of liver and estimate the egg granuloma size on the slide of liver.Results The numbers of egg node of rSj14-3-3 group,rSj14-3-3/SjGST group and control group were 6.72±1.14,5.89±1.03 and 21.05±1.26 respectively.The former two were decreased by 68.08% and 72.02%;the mean granuloma sizes were decreased by 35.23% and 46.13% with 178.12±32.18μm and 148.13±29.65μm in two immunized groups and 275.00±38.21μm in control group.Conclusion The two recombinant antigens rSj14-3-3 and rSj14-3-3/SjGST have a rather good effect on anti-granuloma formation of Schistosomiasis japonica.