Determination of Three Flavonoids in Lespedeza virgata by RP-HPLC

Objective:To establish a quantification method for determination of quercetin,rutoside and kaempferol in Lespedeza virgata by HPLC.Method:RP-HPLC was applied on the determination of quercetin,rutoside and kaempferol in L.virgata with C18 column(4.6 mm × 250 mm,5 μm),DAD detector,and a mixture of acetonitrile-0.2% phosphoric acid(60∶ 40) as a mobile phase with the flow rate at 1.0 mL.min-1.Three flavonoids were detected at 251 nm,column temperature was set at 35 ℃.Result:Quercetin,rutoside and kaempferol were linear in the range of 0.210-4.19,0.084-1.68,0.066-1.33 μg.The average recovery of rutoside,quercetin and kaempferol was 99.48%,98.53% and 99.33% with RSD(n = 5) 0.51%,1.35% and 2.13% respectively.Conclution:The method is accurate and sensitive,which can be used rapidly for the content determinatinon of rutoside,quercetin and kaempferol from L.virgata.It can be applied to the quality control of this crude drug.