Usefulness of PCR Technique in Identification of Alternaria Alternata Allergen Extracts

Objective To produe the allergen extracts with Alternaria alternarta while were identified by PCR technique.Methods Alternaria alternata was collected from air and cultured under 26℃,identified by PCR technique.Result The fungus that was collected from air and identified as Alternaria alternata by morphology,its 26srDNA was amplified by PCR,resulting a 580 bp band,after sequencing,under microscope there were 543 bases those could be identical and consistent to the Alternaria alternata data from internet based on the result of blast comparison,corresponding rate is 99%.Conclusion We succeeded in collecting and producing Alternaria alternata.