Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat

Objective To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods Lysis buffer of different volume was taken to extract brain tissue proteins of rat,and different protein quantities(1 mg,2 mg,3 mg)were taken to establish a two-dimensional electrophoresis.Coomassie brilliant blue was applied to stain protein,and patterns were analyzed.Results With a molecular mass between 6.5~200 KDa and isoelectric points(pI)from 3~10,1 mg proteins obtained 574 protein spots,2 mg 798,and 3 mg 803.Conclusion A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.