Clinical Significance of Detection of Multidrug Resistance Gene1 Expression in Acute Leukemia

Objective: To study diagnosing method of multidrug resistance with leukemia combining clinical chemotherapy effect. Methods:Intracellular medication concentration was examined in patients with leukemia by flow cytometry and relative expression levels of mdr1 which was estimated by RNA in situ hybridization. Results: Average intracellular DNR accumulation in refractory and relapsing patients(4/12 mdr1 expression negative) was (44.80±7.74)%, but that in newly diagnosed and treated patients(10/10 mdr1 expression negative) with remission was (80.01±2.10)%. Intracellular DNR accumulation in newly diagnosed and treated patients with remission were significantly higher than that in refractory and relapsing patients ( P 0.01). Conclution: Mdr1 detection by the two above mentioned methods could filter the type of multidrug resistance in patients with leukemia and could help to project the clinical chemotherapy schedule.