The protective effect of compound N-2035on focal brain ischemia-reperfusion in jury in rats

Objective:To investigate the protective effect of compound N-2035obtained from puerarin,a kind of isoflavone that was extracted from Chinese traditional medicine on focal brain is-chemia-reperfusion injury in rats.Methods:The models of focal brain ischemia reperfusion injury by middle cerebral artery occlusion(MCAO)were established in Wister rats.Cerebral edema was measured by dry /wet mass method and intracellular calcium and the expression of bcl-2was examined by flow cytometer.Re sults:N-2035in a dose of40mg /kg positively relieved cerebral edema;each dose de-creased intracellular calcium and increased the expression of bcl-2.Con clusion:Compound N-2035can relieve brain damage induced by focal ischemia /reperfusion in rats.The mechanism may be related with the decrease of intracellular calcium and the increase of the expression of bcl-2.