Effect of haikunshenxi on the protection for kidney and the monocyte chemoattractant protein-1 and mRNA expression in kidney of diabetic rats

Objective:To study the expression of monocyte chemoattractant protein-1(MCP-1) and mRNA in the renal tubules of STZ-induced diabetic nephropathy rats.Methods:Sixty SD rats were randomly divided into two groups:normal control(n=10) and diabetic nephropathy group(n=50).Diabetes was induced by intraperitoneal injection of STZ.And the diabetic rats were randomly divided into model group,irbesartan treatment group,haikunshenxi at low and high doses treatment group.Twenty four-hour urine volume,24-hour urinary protein excretion were examined at weeks 4,8,and 12.All rats were sacrificed at week 12;body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge were improved by haikunshenxi preparation.The expression of MCP-1 and mRNA in rat kidney were detected respectively by immunohistochemistry and in situ hybridization.Results:The expression of MCP-1and mRNA in haikunshenxi treatment group was depressed greatly in the renal tubular epithelial cell and renal interstitial cell(P0.01).Conclusions:Haikunshenxi can inhibit the overexpression of MCP-1 and mRNA in kidney of diabetic rats and delay the progress of diabetic nephropathy.