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2006•Acta Nutrimenta SinicaRequires access

RAPID DETERMINATION OF TOTAL VITAMIN E IN LYCOPENE OLEORESIN BY SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Jingcheng Zhao

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Abstract

Objective:To establish a rapid determination method of total VE in lycopene oleoresin.Method:By eliminating saponifiable matters and strong polar materials from the sample,VE was concentrated efficiently by PRP-6 SPE with 90% ethanol/water and 100% ethanol as elution solvents consecutively.The removal of polar materials was satisfactory.VE was determined by HPLC.Results:The linear regression was carried out,the relative coefficient r=0.9998 and 99.85～99.95 % recovery of VE were achieved.Conclusion:A simple and rapid VE determination method with good sensitivity is developed.

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What this paper is about

Objective:To establish a rapid determination method of total VE in lycopene oleoresin.Method:By eliminating saponifiable matters and strong polar materials from the sample,VE was concentrated efficiently by PRP-6 SPE with 90% ethanol/water and 100% ethanol as elution solvents consecutively.The removal of polar materials was satisfactory.VE was determined by HPLC.Results:The linear regression was carried out,the relative coefficient r=0.9998 and 99.85～99.95 % recovery of VE were achieved.Conclusion:A simple and rapid VE determination method with good sensitivity is developed.

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Available abstract

Objective:To establish a rapid determination method of total VE in lycopene oleoresin.Method:By eliminating saponifiable matters and strong polar materials from the sample,VE was concentrated efficiently by PRP-6 SPE with 90% ethanol/water and 100% ethanol as elution solvents consecutively.The removal of polar materials was satisfactory.VE was determined by HPLC.Results:The linear regression was carried out,the relative coefficient r=0.9998 and 99.85～99.95 % recovery of VE were achieved.Conclusion:A simple and rapid VE determination method with good sensitivity is developed.

Key concepts: Chromatography, Lycopene, Elution, High-performance liquid chromatography, Chemistry, Oleoresin, Extraction (chemistry), Solid phase extraction

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RAPID DETERMINATION OF TOTAL VITAMIN E IN LYCOPENE OLEORESIN BY SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY — Research Paper | ScholarLens